Associate Professor of Biological Chemistry
Johns Hopkins University School of Medicine
725 N. Wolfe St.
Office Phone: 410-955-4621
Human amyloid A protein structure and pathophysiology
We are using molecular techniques to study human pathophysiology and have concentrated on the human serum amyloid A (SAA) gene/protein family. These small acute phase proteins are synthesized in response to trauma, infection, inflammation and neoplasia and their fragments form tightly ordered fibrillar arrays responsible for clinical amyloidosis. One of these proteins appears to be a mediator of inflammatory joint destruction. Another has prominent expression in the brain. Having studied the responsible gene, we are now examining the proteins themselves and developing systems to determine their structure using NMR and crystallograpy.
<p>1. Sack, G.H., Jr. (1996) Clinical Applications of Molecular Genetics. The Principles and Practice of Medicine (John D. Stobo, David B. Hellmann, Paul W. Ladenson, Brent G. Petty and Thomas A. Traill, editors) 23rd edition, Appleton & Lange, Stamford, cahpter 10.2, pp 690-698.</p>
<p>2. Sack, G.H., Jr. (1997) Molecular Genetics in the Diagnosis of Canine Diseases. Advances in Veterinary Science and Comparative Medicine 40, 103-117.
<BR><A HREF="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9395730&dopt=Abstract ">PubMed Reference </a></p>
</p>3. Meeker, A.K. and Sack, G.H., Jr. (1998) A fusion protein between Serum Amyloid A and Staphylococcal Nuclease – Synthesis, purification and structural studies. Proteins:
Structure, Function and Genetics 30, 381-387.
<A HREF="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9533622&dopt=Abstract ">PubMed Reference </A></p>
</p>4. Sack, G.H., Jr. (1999) Medical Genetics McGraw-Hill, New York. 286 pp.</p>
<p>5. Provost, T.T. and Sack, G.H., Jr. (2001) Porphyrias in Cutaneous Medicine - Cutaneous Manifestations of Systemic Disease Thomas T. Provost and John A. Flynn (Editors) B.C. Decker, Hamilton, chapter 39, pp.487-496.</p>